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第18号公告板 - Spring 2004
Parasitology- No Category
TAKASHIMA M, OHMI H, WATANABE T, OKAMOTO K, KANOE M, NAGAI S.
Attempts to separate female Ascaris suum antigen and to investigate its partial characterization.
Veterinary Journal vol. 165 ( 2 ): p.164-168 (2003)
The location and separation of Ascaris suum antigen for serological testing was investigated. The antigenic constituent was rich in the ovary of the adult worm and was obtained by dialysis with 50% ammonium sulfate saturated solution. Sodium dodecyl sulfate polyacrylamide gel electrophoresis and immunoblotting analysis demonstrated that the heat labile antigenic preparation showed one major and seven faint bands. The major band seemed also to be a glycoprotein. The sera from pigs with/without hepatic milk spot showed relatively high precipitation titres, while, those from the specific pathogen free pigs manifested low titres. 13 ref.
