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第22号公告板 - Summer 2005
MiscelleanousImmunology
HAMMOND JM, JOHNSON MA
Porcine adenovirus as a delivery system for swine vaccines and immunotherapeutics.
Veterinary Journal, 2005, Volume 169, N°1, 17-27
Several experiments have been carried out with adenovirus as delivery vectors in swine and this new type of vaccine is increasingly generating interest. The idea is to insert into the genome of an adenovirus the genes encoding for the immunogenic antigens of a pathogen and administer the recombinant adenovirus in order to generate a protective immune response in the host. Adenoviruses have a low pathogenicity and a large worldwide-distributed variety of serotypes with different virulence and tissue tropism. In addition, adenoviruses show a high grade of replication in tissue cell cultures and the costs of production of a potential vaccine would be low. Such recombinant vaccines can be administered parenterally or by the oral route in feed or drinking water. The protocol and the results of two experiments performed with recombinant porcine adenovirus (rPADV) respectively expressing gp55 of classical swine fever virus, and gD of Aujeszky’s disease virus are described. Both vaccines provided a complete protection at challenge (no disease, no lesions). Moreover, the recombinant adenovirus was not isolated again in challenged animals indicating that the problems of shedding can be ruled out. The use of recombinant adenovirus as a delivery vector for therapeutic molecules was also investigated using genes encoding for porcine cytokines (IFNγ, granulocyte colony stimulating factor (G-CSF)). The rPADV-cytokine treatment of swine resulted highly promising and the authors consider it as a reliable replacement for in-feed antibiotics in future.
